Team:Warsaw/Stage1/ExpressIt

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• Team
• Attribution
• Sponsors
• Acknowledgemets

• Idea
• Background
• Promoters Measured
• RBSes Measured
• ExpressIt! Vectors
• Modeling

• Idea
• Background
• Design
• Results

• Idea
• Background and Design
• Results
• Gallery of microphotographs

• Idea
• Background
• Design
• Results
• Modeling

• Parts
• Labbook

• Licensing of biological parts
• Syntetic biology in Poland
• Synthetic BioLectures gallery
• iGEM survey

• About Biobrick Manager
• See Biobrick Manager

ExpressIt! - family of expression vectors with defined strength

Key features:

• 10 different measured RBS variants
• each variant in both constitutive (J23100 driven) and inducible (pT7 driven) version
• just one step to clone any protein coding BioBrick and you have expression construct ready
• wide spectrum of expression strengths
• ampicillin resistance (new variants of E.coli BL21 are chloramphenicol resistant)
• high copy ColE1 ori (based on pSB1A2 vector)
• terminator included in vector sequence

Relative strength of ExpressIt! vector family: