Team:Warsaw/Stage2/Design

Revision as of 12:28, 23 October 2010 by Milena igem (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

• Team
• Attribution
• Sponsors
• Acknowledgemets

• Idea
• Background
• Promoters Measured
• RBSes Measured
• ExpressIt! Vectors
• Modeling

• Idea
• Background
• Design
• Results

• Idea
• Background and Design
• Results
• Gallery of microphotographs

• Idea
• Background
• Design
• Results
• Modeling

• Parts
• Labbook

• Licensing of biological parts
• Syntetic biology in Poland
• Synthetic BioLectures gallery
• iGEM survey

• About Biobrick Manager
• See Biobrick Manager

Safety

The safety system we designed and measured consists of the parts specified: T7 promoter + MinC + RBS B0034 on pSB plasmid, studied in E.coli BL21 strain.

<partinfo>BBa_K299807 DeepComponents</partinfo> <partinfo>BBa_K299808 DeepComponents</partinfo>

We measured our construct in vivo, in the expression system, using strong T7 promoter, IPTG induced. The functionality of our safety element describes optical density of E.coli (OD) and the number of colony forming units/ ml (cfu/ml) in IPTG-induced innoculates BL21 carrying our construct.