Team:Kyoto/Parts

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Contents

Parts

Original

List

<groupparts>iGEM010 Kyoto</groupparts>

<partinfo>K358000</partinfo>

<partinfo>BBa_K358000 short</partinfo>

The lac promoter will be repressed and not induce GFP in the presence of lacI. We measured the strength of fluorescence with low copy plasmid vector, pSB4K5.

<partinfo>K358001</partinfo>

<partinfo>BBa_K358001 short</partinfo>

constitutive promoter[J23101] with GFP on low plasmid[pSB4K5]

<partinfo>K358004</partinfo>

<partinfo>BBa_K358004 short</partinfo>

This part causes cell death. It contains holin/antiholin, endolysin and Rz/Rz1 genes.

<partinfo>K358006</partinfo>

<partinfo>BBa_K358006 short</partinfo>

Lambda lysis cassette[BBa_K358004] with double terminator[BBa_B0015].

<partinfo>K358010</partinfo>

<partinfo>BBa_K358010 short</partinfo>

It codes SΔTMD1 as the anti-killer gene, which is derived from λ phage DNA and against the lambda lysis cassette[BBa_K358005]: the killer gene.

<partinfo>K358012</partinfo>

<partinfo>K358013</partinfo>

<partinfo>K358015</partinfo>

<partinfo>K358016</partinfo>

<partinfo>K358017</partinfo>

<partinfo>K358018</partinfo>

<partinfo>K358019</partinfo>

<partinfo>K358020</partinfo>

<partinfo>K358021</partinfo>

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BioBrick

Parts

NameDescriptionWell*1PlasmidResistanceInsert LengthVector Length
<partinfo>J23100</partinfo>Constitutive promoter family member1-18-C<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>J23105</partinfo>Constitutive promoter family member1-18-M<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>J23116</partinfo>Constitutive promoter family member1-20-M<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>R0011</partinfo>Promoter (lacI regulated, lambda pL hybrid)1-6-G<partinfo>pSB1A2</partinfo>Ampicillin552079
<partinfo>B0015</partinfo>Double terminator (<partinfo>B0010</partinfo>-<partinfo>B0012</partinfo>)1-23-L<partinfo>pSB1AK3</partinfo>Ampicillin, Kanamycin1293189
<partinfo>E0840</partinfo>GFP generator1-12-O<partinfo>pSB1A2</partinfo>Ampicillin8782079
<partinfo>E0240</partinfo>GFP generator1-12-M<partinfo>pSB1A2</partinfo>Ampicillin8762079
<partinfo>I20260</partinfo>Measurement Kit Test of <partinfo>J23101</partinfo>2-17-F<partinfo>pSB3K3</partinfo>Kanamycin9192750
<partinfo>J06702</partinfo>mCherry, bacterial with RBS and forward terminator2-8-E<partinfo>pSB1A2</partinfo>Ampicillin8692079
<partinfo>pSB3K3</partinfo>RFP Coding Device1-5-E<partinfo>pSB3K3</partinfo>Kanamycin10692750
<partinfo>pSB4K5</partinfo>Low copy BioBrick standard vector1-5-G<partinfo>pSB4K5</partinfo>Kanamycin10693419
<partinfo>pSB1C3</partinfo>High copy BioBrick assembly plasmid<partinfo>pSB1C3</partinfo>Chloramphenicol2072
  • *1 "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.

Primers

NameDescriptionSequence
<partinfo>G00100</partinfo>Forward primer for sequencing/amplifying BioBrick parts (VF2)tgccacctgacgtctaagaa
<partinfo>G00101</partinfo>Reverse primer for sequencing/amplifying BioBrick parts (VR)attaccgcctttgagtgagc

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