Team:UCL London/Our BioBricks

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UCL IGEM 2010

RETURN TO IGEM 2010

NEW PARTS CHARACTERISED

The following table is that of all the Biobricks and parts that we have used over the course of iGem 2010. This does not necessary mean that they were all incorporated into the final product!

Part Code Part Name Location Backbone Length(bp)
R0010 PROMOTER (lacI Regulated) Plate1_1D pSB1A2 200
R0010 PROMOTER (lacI Regulated) Plate1_1D pSB1A2 200

EXISTING PARTS/DEVICES CHARACTERISED

Part Code Part Name Location Backbone Length(bp)
BBa_K239011 Stress Light GFP Anaerobic Metabolism Detector 2 972
K239009 GFP test devise for Spy promoter 1045
K239015 GFP test devise for DegP promoter 1116

Parts Submitted

Part Name Part Type Description Designer Length(bp)
BBa_K301001 Regulatory Tac promoter, hybrid derived from trp and lac promoters Xiang Chen 73
BBa_K301001 Device pTAC with RFP reporter Xiang Chen 942


Check out our parts page to see the parts submitted in more detail [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=UCL_London&Done=1|UCL_London 2010 iGEM Team Parts].

OTHER PARTS/DEVICES USED

Part Code Part Name Location Backbone Length(bp)
R0010 PROMOTER (lacI Regulated) Plate1_1D pSB1A2 200
K239005 Nark Eurogentec Amp 139
K239006 mNark Eurogentec Amp 89
R0079 Promoter (LasR & PAI regulated) Plate1_19P pSB1A2 936
K274100 VRDP Plate3_6H pSB1A2 3385
B0015 Double Terminator Plate1_23L pSB1AK3 129
pSB1K3 Kanamycin Resistance Plate1_5A pSB1K3+J04450 insert 2206
pSB1T3 Tetracycline Resistance Plate 1_7A pSB1T3+J04450 insert 2463
pSB1C3 Chloramphenicol Resistance Plate 1_3A pSB1C3+J04450 insert 2072
R0011 Promoter (lacI regulated, lambda pL hybrid) Plate1_6G pSB1A2 55
B0034 RBS Plate1_2M pSB1A2 12
C0079 lasR activator from P. aeruginosa PAO1(+LVA) Plate1_14J pSB2K3 756
pSB1AK3 Amp+Kana Plate1_11A +J04450 insert 3189
pSB1AT3 Amp+Tetra Plate1_13A +J04450 insert 3446
pSB1AC3 Amp+Chlora Plate1_9A +J04450 insert 3055
K274200 CrtEBIY with rbs (Orange) Plate3_6L pSB1A2 4555
K274002 Purple Plate3_12B pSB1T3 7345
K274003 Dark Green Plate3_20H pSB1K3 6032
row 15, cell 1 row 15, cell 2 Plate3_20D pSB1T3 6032
K274004 Light Green Plate3_20J pSB1K3 6200
Plate3_22F pSBT3 6200
I712069 RBS+T7 RNAP Plate2_13K pSB1AK3
I719005 T7 promoter Plate1_15N pSB1A2
I712074 T7 strong pro Plate1_6N pSB1AK8
I13507 RBS+RFP+T Pate1_22O pSB1A2 861


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