Team:TU Delft/21 June 2010 content
From 2010.igem.org
We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.
Lab work
Restriction digestion of plasmid backbone pSB1T3:
pSB1T3 (~1,0 μg) | 2,5 μL |
Buffer H (Roche) | 2,0 μL |
EcoRI | 1,0 μL |
PstI | 1,0 μL |
H2O | 13,5 μL |