"
Team:SDU-Denmark/primers
From 2010.igem.org
Revision as of 13:43, 21 October 2010 by MarieBrønsNørgaard (Talk | contribs)
Primers
On this page we list all the primers we designed and used during the summer. All primers were kindly provided by [http://www.dna-technology.dk/ DNA Technology].
| Primer name | Sequence | Tm (C) |
| FlhDC fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAA-3' | 88.3 |
| FlhDC rv | 5'-GTTTCTTCCTGCAGCGGCCGCTACTATTAAACAGCCTGTACTCTCTGTTC-3' | 88.3 |
| FlhDC mutation fw* | 5'-TTGGCAGCTTTGCCCGCAGCTTATG-3' | 61.0 |
| FlhDC mutation rv* | 5'-CATAAGCTGCGGGCAAAGCTGCCAA-3' | 61.0 |
| Photosensor fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTGGGACTTACGACCTC-3' | 73.1 |
| Photosensor rv | 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGAAGGTTTCCCAGTTCGCATC-3' | 73.9 |
| NinaB fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' | 73.1 |
| NinaB rv | 5'-GTGTCTTCCTGCAGCGGCCGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' | 72.4 |
| NinaB 2 fw** | 5'-CCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' | 68.1 |
| NinaB 2 rv** | 5'-CGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' | 65.6 |
(*) Mutation primers were designed to introduce a silent mutation at position 822 in the operon to eliminate an illegal PstI site.
(**) Due to the formation of hairpin structures of primers ninaB fw and ninaB rv, new primers were designed containing only the innermost restriction sites (XbaI and SpeI).
http://igem.sdu.dk/wp-content/uploads/sponsor-sdu.png http://igem.sdu.dk/wp-content/uploads/sponsor-fermentas.png http://igem.sdu.dk/wp-content/uploads/sponsor-dnatech.png 
Insert clever content here.
