September 1

pREP41 vector has arrived.

-> we will do E.coli transformation and increase its number.

pREP42-GFP vector has arrived (in E.coli)

-> will incubate a day and do spreading for further experiment.

Making culture media

1. 500mL LB broth + agar 1.5% (7.5g)
2. Do auto clave
3. Cool it down until it reaches 50~60℃
4. Add ampicillin(1000x 100mg/mL) and stir
5. putting out bubbles on culture media
6. pouling on petri dish and wait for 1 hour

Culture

1. Competent cell + DN10A and leave it in ice for 30min
2. Do heat shock for 45 sec and store it in ice for 2min
3. Stabilize it in incubator for 30 to 40min
4. Do spreading

September 2 ~ September 21

We designed PCR schedules and do PCR experiment. When PCR result was not satisfied, then we repeated PCR experiment. There are PCR details on PCR experiment menu.