"
Team:TU Delft/20 September 2010 content
From 2010.igem.org
Meeting minutes
- Hugo - Maybe some issues with the measurements over the weekend – Thursday on Friday measurement went really well, those results can be uploaded. This was done with different concentrations of hexane
- Nadine – Salt tolerance, measured over the weekend (with LB and M9, concentrations of 0.6-0.9M NaCl)
- Hugo is going to do the characterization of alkane degradation; Friday, Ramon will help Hugo in the lab with GC measurements
- Do a digestion check of 017?
- Eva: 1C3 issues – grow and isolate again
- Luke: Pathway maps have been made coupled to MFA for on the wiki, but he should also explain the graphs in words (not only graphs)
- It should be good to have a page that summarizes all the results, on which you can click to more information/details
- Jelmer: Software tool – almost done, results will be linked to prefoldin
- Nadine: Survey on the 25th, no plan yet for where, but questions already thought up, but Dirk Stemerding will
Alkane degradation
For some reason, 009 and 010 just don't want to get into one plasmid. As a last resort we'll try every possible way to get the two together.
Digestions:
| Lane # | BioBrick | Cut with | Extra | Buffer | BSA | Gel ok? |
| 0 | Smartladder (5μl) | |||||
| D 1 | 009A | EcoRI, SpeI | AseI | Buffer 2 (NEB) | 1x BSA | OK |
| D 2 | 009A | SpeI, PstI | Phosphatase | Buffer 2 (NEB) | 1x BSA | OK |
| D 3 | 009A | SpeI, PstI | none | Buffer 2 (NEB) | 1x BSA | OK |
| D 4 | 009A | EcoRI, XbaI | none | Buffer 2 (NEB) | 1x BSA | OK |
| D 5 | 009A | XbaI, PstI | AseI | Buffer 2 (NEB) | 1x BSA | OK |
| D 6 | 010A | EcoRI, SpeI | AseI | Buffer 2 (NEB) | 1x BSA | OK |
| D 7 | 010A | SpeI, PstI | none | Buffer 2 (NEB) | 1x BSA | OK |
| 8 | 010A | EcoRI, XbaI | Phosphatase | Buffer 2 (NEB) | 1x BSA | OK |
| D 9 | 010A | EcoRI, XbaI | none | Buffer 2 (NEB) | 1x BSA | OK |
| D 10 | 010A | XbaI, PstI | AseI | Buffer 2 (NEB) | 1x BSA | OK |
Ligations:
| '# | 'Piece 1 | Piece 2 |
| 028 L1 | D1 | D8 |
| 028 L2 | D1 | D9 |
| 028 L3 | D2 | D10 |
| 028 L4 | D3 | D10 |
| 028 L5 | D4 | D6 |
| 028 L6 | D5 | D7 |
