"

From 2010.igem.org

Blue Light Producer

13/7/2010

Empty edinbrick vector for control.

• LuxAB + edinbrick ligation.

28/7/2010

an update on LuxAB:

• The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
• Still have some digested LuxAB-ediI so re-did ligation and transformation.

Gel Pic- minipreps

Lane 1&15	Lane 2	Others
Ladder	EdiI	LuxAB

GEL PIC

Lane 1	Lane 2 +3	Lanes 3-6
Ladder	EdiI	LuxAB

• The ligation had the same gel characteristics as previous luxAb-ediI ligations, e.x. it isn't visivle- not hopeful of this transformation working

29/7/2010

12/8/2010 update on LumP:

• still getting pink/red colonies from transformants
• single digests give a band of about 3kb
• double digests give a band of about 2.5 kb -highly confusing
• and also, no sign on RFP despite red/pink growth.
• Vector wa ssequenced and LumP is definately there.

gel pohot no x and y + description.