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Cat-O2-lase Protein

The XylE gene, originating from the TOL plasmid of Pseudomonas putida, was the choice for the output signal mediator of our system. The system is based on production of catechol 2,3-dioxygenase enzyme. Colonies of cells that express xylE become yellow within seconds after selection plates are sprayed with catechol, a colorless substrate that is converted by CatO2ase to the yellow product, 2-hydroxymuconic semialdehyde. This reporter system is ideal for our project as it is very fast, gives a visual output by naked eye and the substrate it uses is very cheap.

Characterization of CatO2lase protein

The CatO2lase protein, except as an ideal output signal of our engineered bacterium