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Lab notes (10/4 - 10/10)

Contents 1 Lab notes (10/4 - 10/10) 1.1 Flagella Group 1.2 Retinal 1.2.1 PCR amplification of K343005 with dobbelt terminator

Flagella Group

Retinal

PCR amplification of K343005 with dobbelt terminator

Date: 8/10
Done by: Marie
Methods: PCR
Protocols: CP1.1[1]
Notes:
8 colonies were selected of colony 63 form colony PCR.
VF2 and VR was used as primers in the PFU PCR
PCR Program:

Start	94  C	2 min
Denaturing	94 C	1 min
Annealing	55 C	1 min
Elongation	72 C	2:30 min
Goto2	rep	29x
End	72 C	5 min
Hold	4 C

Afterwards a gel was run.