Team:Harvard/flavor/parts
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parts and primers
Primers
Name | Description | Sequence |
J45004_F | Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer | |
J45004_R | Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer | |
J45017_F | Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer | |
J45017_R | Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer | |
NOSterm_BB_F | Plant specific terminator forward primer | |
NOSterm_BB_R | Plant specific terminator reverse primer | |
Stop_NOSterm_BB_F | NOSt plant specific terminator with stop codon on end | |
pENTCUP2_BB_F | Plant specific promoter forward primer | |
pENTCUP2_BB_R | Plant specific promoter reverse primer |
Parts
Name | Description | Sequence |
pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. | |
NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. | |
NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on end. | |
Brazzein | Sweet-tasting protein. 54 amino acid sequence. | |
Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | ATGCAAGATAAGTGTAAAAAAGTGTATGAGAACTATCCTGTGAGTAAATGCCAATTGGCAAACCAGTGCAATTATGATTGTAAACTCGATAAGCACGCTAGGAGTGGAGAGTGTTTCTATGATGAGAAGAGGAACCTCCAGTGTATCTGTGATTATTGTGAGTAT |
Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | |
Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | |
Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | |
Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. |