Team:UPO-Sevilla/Biobricks/Devices
From 2010.igem.org
Devices
In this section all devices we designed at the beginning of the summer are shown in a schematic way. You can see parts which make up each device and required inputs to perform each particular output. Another important information is the use of each device. Finally we present the optimized assembly strategy we followed to try to build these devices.
As you can see, we wanted to assembly a lot of parts, too much devices for our first time. This is why we had to focus in some devices and to forget assembling others. You will see a yellow star in the upper left corner of the prioritized devices. These are the devices we finally assembled.
Device 1. Type: protein generator
![Bacterial Crowding Device 01](https://static.igem.org/mediawiki/2010/6/66/BacterialCrowdingDevice01.png)
Notes: This device includes all Prh system proteins we synthesized using Mr. Gene services. Finally it was not built.
Device 2. Type: protein generator
![Bacterial Crowding Device 02](https://static.igem.org/mediawiki/2010/1/16/BacterialCrowdingDevice02.png)
Notes: This device includes all Fec system proteins we wanted to synthesize by PCR and SDM methods. We decided not to build this divice due to the fact that it already exists in E. coli K12 strain.
Device 3. Type: protein generator
![Bacterial Crowding Device 03](https://static.igem.org/mediawiki/2010/0/04/BacterialCrowdingDevice03.png)
Notes: This device was created to perform an hybrid Fec/Prh system. Instead of it we built device 17 which also allows to use circuit 3.
Device 4. Type: protein generator
![Bacterial Crowding Device 04](https://static.igem.org/mediawiki/2010/d/dd/BacterialCrowdingDevice04.png)
Notes: We were not confident of circuit 4 function and we had not time to lose. That means that we did not assembly required parts for device 4.
Device 5. Type: reporter
![Bacterial Crowding Device 05](https://static.igem.org/mediawiki/2010/5/54/BacterialCrowdingDevice05.png)
Notes: We focused in circuit 3, which uses PfecA promoter, so we did not continue building any device with PprhJ promoter; although we synthesized PprhJ promoter using Mr. Gene services.
Device 6. Type: reporter
![Bacterial Crowding Device 06](https://static.igem.org/mediawiki/2010/a/a8/BacterialCrowdingDevice06.png)
Notes: PRIORITIZED DEVICE!
Device 7. Type: Protein generator/Signal sender
![Bacterial Crowding Device 07](https://static.igem.org/mediawiki/2010/e/ef/BacterialCrowdingDevice07.png)
Notes:
Device 8. Type: Protein generator/Signal sender
![Bacterial Crowding Device 08](https://static.igem.org/mediawiki/2010/a/a4/BacterialCrowdingDevice08.png)
Notes: PRIORITIZED DEVICE!
Device 9. Type: Protein generator/Signal sender
![Bacterial Crowding Device 09](https://static.igem.org/mediawiki/2010/0/05/BacterialCrowdingDevice09.png)
Notes:
Device 10. Type: Protein generator/Signal sender
![Bacterial Crowding Device 10](https://static.igem.org/mediawiki/2010/d/de/BacterialCrowdingDevice10.png)
Notes: Finally we chose aspartate like the best attractant for E. coli. This is why Glutamate Synthase divices were not going to continue building.
Device 11. Type: Protein generator/Signal sender
![Bacterial Crowding Device 11](https://static.igem.org/mediawiki/2010/d/d2/BacterialCrowdingDevice11.png)
Notes: Finally we chose aspartate like the best attractant for E. coli. This is why Glutamate Synthase divices were not going to continue building.
Device 12. Type: Protein generator/Signal sender
![Bacterial Crowding Device 12](https://static.igem.org/mediawiki/2010/f/fd/BacterialCrowdingDevice12.png)
Notes: Finally we chose aspartate like the best attractant for E. coli. This is why Glutamate Synthase divices were not going to continue building.
Device 13. Type: Protein generator/Signal sender
![Bacterial Crowding Device 13](https://static.igem.org/mediawiki/2010/f/fd/BacterialCrowdingDevice13.png)
Notes:
Device 14. Type: Protein generator/Signal sender
![Bacterial Crowding Device 14](https://static.igem.org/mediawiki/2010/1/1a/BacterialCrowdingDevice14.png)
Notes: PRIORITIZED DEVICE!
Device 15. Type: Protein generator/Signal sender
![Bacterial Crowding Device 15](https://static.igem.org/mediawiki/2010/6/61/BacterialCrowdingDevice15.png)
Notes:
Device 16. Type: Constitutive protein generator/reporter
![Bacterial Crowding Device 16](https://static.igem.org/mediawiki/2010/5/5e/BacterialCrowdingDevice16.png)
Notes:
Device 17. Type: Protein generator
![Bacterial Crowding Device 17](https://static.igem.org/mediawiki/2010/e/e8/BacterialCrowdingDevice17.png)
Notes: PRIORITIZED DEVICE!
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