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User contributions
From 2010.igem.org
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- 19:12, 23 October 2010 (diff | hist) Team:Northwestern/Project/Chitin Synthesis
- 19:03, 23 October 2010 (diff | hist) Team:Northwestern/Notebook (→10/22/10)
- 19:00, 23 October 2010 (diff | hist) Team:Northwestern/Project/Chitin Synthesis
- 18:59, 23 October 2010 (diff | hist) Team:Northwestern/Project/Chitin Synthesis
- 18:51, 23 October 2010 (diff | hist) Team:Northwestern/Project/Chitin Synthesis
- 18:36, 23 October 2010 (diff | hist) Team:Northwestern/Safety
- 18:34, 23 October 2010 (diff | hist) Team:Northwestern/Project/Lac
- 18:33, 23 October 2010 (diff | hist) Team:Northwestern/Project/Apoptosis (top)
- 18:30, 23 October 2010 (diff | hist) Team:Northwestern/Project/Chassis (top)
- 18:30, 23 October 2010 (diff | hist) Team:Northwestern/Project/Chassis
- 18:28, 23 October 2010 (diff | hist) Team:Northwestern/Project/Modeling
- 18:27, 23 October 2010 (diff | hist) Team:Northwestern/Project/Modeling
- 18:26, 23 October 2010 (diff | hist) Team:Northwestern/Project/Modeling
- 18:12, 23 October 2010 (diff | hist) Team:Northwestern/Project/Apoptosis
- 03:00, 23 October 2010 (diff | hist) Team:Northwestern/Project
- 02:51, 23 October 2010 (diff | hist) Team:Northwestern/Project
- 02:28, 23 October 2010 (diff | hist) Team:Northwestern/Project
- 01:41, 23 October 2010 (diff | hist) Team:Northwestern/Acknowledgements
- 01:41, 23 October 2010 (diff | hist) Team:Northwestern/Acknowledgements (→NU Faculty and Affiliates)
- 01:31, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
- 01:30, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
- 01:28, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
- 01:28, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
- 01:27, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/LIVE/DEAD® BacLight - Bacterial Viability Kit (New page: '''Kit Components''' *Component A, DMAO: 2x100 µL, 5 mM in DMSO *Component B, EthD-III: 2x150 µL, 2 mM in DMSO '''Preparation of Live and Dead Bacterial Suspensions as Controls''' #Gro...) (top)
- 01:27, 23 October 2010 (diff | hist) Team:Northwestern/Protocol/Quikchange (from primers to colonies! (top)
- 01:27, 23 October 2010 (diff | hist) Team:Northwestern/Protocol/PDNA (top)
- 01:25, 23 October 2010 (diff | hist) Team:Northwestern/Protocol/Kit to Stock Plasmid (top)
- 01:25, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Methanol Fixation (New page: # Rinse slide with ethanol and flame # Rinse slide with PBS and dry with KimWipe # Apply cell sample to microscope slide and let air dry # Submerge in -20C absolute methanol for 5-10 min #...) (top)
- 01:24, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Rhodamine-Conjugated Chitin Probe (New page: ===Methanol Fixation=== # Rinse slide with ethanol and flame # Rinse slide with PBS and dry with KimWipe # Apply cell sample to microscope slide and let air dry # Submerge in -20C absolute...) (top)
- 01:23, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Reagents (New page: Ampicillin - sodium salt Sigma A95180-5G $50.30 Chloramphenicol - Sigma C0378-5G $29.00 Kanamycin - Sigma K4000-5G $59.60 Tetracycline hydochloride - Sigma T3383-25G $47.50 USA Scien...) (top)
- 01:22, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Mini Prep (New page: ==Materials== For purifying plasmid DNA from ''Escherichia coli'' cells, the [http://www1.qiagen.com/Products/Plasmid/QIAprepMiniprepSystem/QIAprepSpinMiniprepKit.aspx Qiagen Spin Minipre...) (top)
- 01:22, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Ligations (New page: '''Reagents''' *T4 DNA ligase *10x T4 DNA Ligase Buffer *Deionized, sterile H2O *Purified, linearized vector (likely in H2O or EB) *Purified, linearized insert (likely in H2O or EB) '''E...) (top)
- 01:21, 23 October 2010 (diff | hist) Team:Northwestern/Protocol/3A Assembly (top)
- 01:21, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/3A Assembly (New page: __NOTOC__ http://ginkgobioworks.com/support/ ==Introduction== 3A assembly (which stands for three antibiotic assembly) is a method for assembling two BioBrick parts and selecting for corr...)
- 01:20, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Plasmid Construction (New page: '''Construction plasmid''' is uncut backbone plasmid DNA from the three different plasmid backbones used for three antibiotic assembly (pSB1C3, pSB1K3, and pSB1T3). Once it is cut with Ec...) (top)
- 01:20, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Restriction Enzyme Digests (New page: ==Materials== *Restriction enzymes ([http://www.neb.com/nebecomm/products/productR0101.asp EcoR I], [http://www.neb.com/nebecomm/products/productR0133.asp Spe I], [http://www.neb.com/nebe...) (top)
- 01:19, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Glycerol Stocks (New page: ==Materials== Reagents -Bacterial culture growing in appropriate liquid medium -Glycerol, 60%, sterile (see Step 1) -nutrient (LB, TSB, etc.) agar plate containing the approp...) (top)
- 01:19, 23 October 2010 (diff | hist) Team:Northwestern/Protocol/LB Media (top)
- 01:17, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Preparing Plates (New page: ==Notes== *(Optional) pre-heat a H2O bath to 55 deg C. *If you don't know how to use the autoclave, ask for a demo. ==Materials== For 1 L of Media: *10 g of Agar (for 1% Agar plates...) (top)
- 01:17, 23 October 2010 (diff | hist) Team:Northwestern/Protocol/LB Media
- 01:16, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/LB Media (New page: To 700mL of deionized water add: Tryptone 10g Yeast Extract 5g NaCl 10g Fill to 1L '''AUTOCLAVE''' Back to [[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</...)
- 01:16, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Preparation of Competent Cells (New page: ==Materials== *Plate of cells to be made competent *TSS buffer *LB media *Ice *Glassware & Equipment *Falcon tubes *500μl Eppendorf tubes, on ice *200ml conical flask *200μl pipetman o...) (top)
- 01:12, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/O/N Culture (New page: '''REMEMBER TO BE STERILE''' *Take 5mL LB and put in a round bottom tube *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube *'''MAKE SURE YOU ADD THE R...) (top)
- 01:11, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Transformation (New page: #Thaw cells on ice and pipette 25uL aliquots into tubes on ice #Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). #*Note: If you are adding small volumes (~1&...) (top)
- 01:11, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/New Part Design(PCR) (New page: The following protocol is for creating a new biobricks part from a protein coding sequence. '''Primer Design''' # Design a forward primer to your new BioBrick part comprised of the BioBri...) (top)
- 01:10, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Ethanol Precipitation (New page: ==Material== * 100% ethanol * 3M sodium acetate at pH 5.2 * -20°C or -80°C freezer * tabletop centrifuge ==Procedure: PREFERRED -20°C Protocol== #Add the following to your sample: #*2....) (top)
- 01:10, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Kit to Stock Plasmid (New page: '''EXTRACTION FROM KIT''' #With a pipette tip, punch a hole through the foil cover into the corresponding well to the Biobrick™-standard part that you want. Make sure you have properly ...)
- 01:09, 23 October 2010 (diff | hist) Team:Northwestern/Protocol/Quikchange (from primers to colonies! (→Primers)
- 01:08, 23 October 2010 (diff | hist) N Team:Northwestern/Protocol/Quikchange (from primers to colonies! (New page: ==Primers== I use [http://mekentosj.com/enzymex/ enzyme x] to work with DNA sequences, it is installed on all the macs and has nice translation and reverse complement functions. Many of ...)
- 01:07, 23 October 2010 (diff | hist) Team:Northwestern/Protocol
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