Team:Yale/Our Project/Notebook

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iGEM Yale

our project

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Lab Notebook: Week 1

  • Monday 6/7--defended project (yay!)
  • Wednesday 6/9--started in lab--got set up & started cultures of DH5alpha and LE392
  • Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran first copper growth assay in plate reader in PM w/three different ODs of each strain(4 hr. protocol)
  • Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--b/c allowed to overgrow?