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From 2010.igem.org

Contents 1 Module I 1.1 Abstract 1.2 Flow chart 1.3 Landing Pad Insertion 1.4 Helper Plasmid(HP) Insertion 1.5 Donor Plasmid(DP) Construction 1.5.1 Strategy 1 1.5.2 Strategy 2 1.6 Donor Plasmid(DP) Insertion & Recombination Induction 1.7 Removal of Helper Plasmid(HP) 2 Module II 2.1 Strategy 1 2.2 Strategy 2 2.3 Strategy 3

Module I

Abstract

Flow chart

Landing Pad Insertion

Helper Plasmid(HP) Insertion

Donor Plasmid(DP) Construction

Strategy 1

Strategy 2

Donor Plasmid(DP) Insertion & Recombination Induction

Removal of Helper Plasmid(HP)

Module II

Strategy 1

Strategy 2

Strategy 3

Cooperation with Macquarie Australia