Team:Osaka/Notebook week3

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Week3:August 8 - August 14

Attendance: Nakamura, Yasumoto

Miniprep of 1-1C
Restriction digests of 2-20H, 2-20J, 1-1D, 1-18F, 1-1C, 1-13D, 1-2M
Gel electrophoresis of digests ("cut check")
- 2-20H, 2-20J, 1-1C -> OK
- 1-1D, 1-18F, 1-13D, 1-2M -> not cut (single band, MW approx. equal to vector + insert)
Transfer of 1-3A, 1-5A colonies to solution culture (repeat)
- Yesterday's inoculated culture mediums contained the wrong antibiotics!
Transformation of <bbpart>BBa_I13521</bbpart>, <bbpart>BBa_I13522</bbpart>, <bbpart>BBa_I13600</bbpart>, <bbpart>BBa_K204031</bbpart>, <bbpart>BBa_K204051</bbpart>, <bbpart>BBa_K204032</bbpart> from last year's stock

Miniprep of 1-3A, 1-5A
Restriction digests of 1-3A, 1-5A
Gel electrophoresis
1. 1st run: 1-3A, 1-5A (newly miniprepped), 1-1D, 1-18F, 1-13D, 1-2M (repeat)
  - 1-3A, 1-5A -> OK; others -> not cut (again)
2. 2nd run: 1-1D, 1-18F, 1-13D, 1-2M (2nd repeat)
  - all parts not cut

Miniprep of last year's parts transformed on Monday
Gel electrophoresis of 1-1D, 1-18F, 1-13D, 1-2M (3rd repeat!!!)
- all 4 not cut... AGAIN
- so far all restriction digests involving XbaI seem to have failed; problem with enzyme stock?
- will try with different set of restriction enzymes next week
Sent miniprepped last year's parts to ECUST team in Shanghai, China