Team:Osaka/Notebook week3

From 2010.igem.org


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Week3 : August 8 - August 14

August 8 (Sun)

Attendance: Nakamura, Yasumoto

  1. Colony check
    • All parts successfully transformed
  2. Transfer to LB culture medium

August 9 (Mon)

  1. Miniprep of 1-1C
  2. Restriction digests of 2-20H, 2-20J, 1-1D, 1-18F, 1-1C, 1-13D, 1-2M
  3. Gel electrophoresis of digests ("cut check")
    • 2-20H, 2-20J, 1-1C -> OK
    • 1-1D, 1-18F, 1-13D, 1-2M -> not cut (single band, MW approx. equal to vector + insert)
  4. Transfer of 1-3A, 1-5A colonies to solution culture (repeat)
    • Yesterday's inoculated culture mediums contained the wrong antibiotics!
  5. Transformation of BBa_I13521, BBa_I13522, BBa_I13600, BBa_K204031, BBa_K204051, BBa_K204032 from last year's stock

August 10 (Tue)

  1. Miniprep of 1-3A, 1-5A
  2. Restriction digests of 1-3A, 1-5A
  3. Gel electrophoresis
    1. 1st run: 1-3A, 1-5A (newly miniprepped), 1-1D, 1-18F, 1-13D, 1-2M (repeat)
      • 1-3A, 1-5A -> OK; others -> not cut (again)
    2. 2nd run: 1-1D, 1-18F, 1-13D, 1-2M (2nd repeat)
      • all parts not cut

August 11 (Mon)

  1. Miniprep of last year's parts transformed on Monday
  2. Gel electrophoresis of 1-1D, 1-18F, 1-13D, 1-2M (3rd repeat!!!)
    • all 4 not cut... AGAIN
    • so far all restriction digests involving XbaI seem to have failed; problem with enzyme stock?
    • will try with different set of restriction enzymes next week
  3. Sent miniprepped last year's parts to ECUST team in Shanghai, China


July
Su Mo Tu We Th Fr Sa
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
Week1 25 26 27 28 29 30 31
August
Su Mo Tu We Th Fr Sa
Week2 1 2 3 4 5 6 7
Week3 8 9 10 11 12 13 14
Week4 15 16 17 18 19 20 21
Week5 22 23 24 25 26 27 28
Week6 29 30 31
September
Su Mo Tu We Th Fr Sa
Week6 1 2 3 4
Week7 5 6 7 8 9 10 11
Week8 12 13 14 15 16 17 18
Week9 19 20 21 22 23 24 25
Week10 26 27 28 29 30
October
Su Mo Tu We Th Fr Sa
Week10 1 2
Week11 3 4 5 6 7 8 9
Week12 10 11 12 13 14 15 16
Week13 17 18 19 20 21 22 23
Week14 24 25 26 27 28 29 30
31