Light sensor: light sensor may have been completed, as Parts A&B&C may have been ligated together. However, we found that the complete part may be questionable, as when we digested them, we find that they are not the right lengths.
ON switch progress: All colonies turned red. . Spooky transcription returs. Will continue w/ part regardless. Also, begin next portion:
I12006-B0034-C0051-B0034-E1010-F1610
To help explain part construction, the composite part of B0034-C0051-B0034-E1010-F1610 will be used: K327001
I12006 will be cut with Spe & Pst; K327001 will be cut with Xba & pst
OFF switch
Starting off with new digestions, attempting with the fast digest procedure
Initiator progress: Ligation of RBS-C0051-B0015 worked!
Week 8
We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!
We would also like to thank and acknowledge: Our Advisors
Marc Facciotti
Ilias Tagkopoulos Technical Guidance
David Larsen
Andrew Yao Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China) cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)
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