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- Light sensor: light sensor may have been completed, as Parts A&B&C may have been ligated together. However, we found that the complete part may be questionable, as when we digested them, we find that they are not the right lengths.
- ON switch progress: All colonies turned red. . Spooky transcription returs. Will continue w/ part regardless. Also, begin next portion:
- I12006-B0034-C0051-B0034-E1010-F1610
- I12006 will be cut with Spe & Pst; B0034-C0051-B0034-E1010-F1610 (also known as K327001) will be cut with Xba & pst
- OFF switch
- Starting off with new digestions, attempting with the fast digest procedure
- I12006 (ES)-K327001 (EX)
- B0034 (ES)-C0052 (EX)-I746352 (SP)-B0034 (XP)-C0040 (ES)-B0015 (EX)
- Initiator progress: Ligation of RBS-C0051-B0015 worked!
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We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!
We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)
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