Team:HokkaidoU Japan/Notebook/August20

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Notebook

Amplified vector by PCR

HokkaidoU Japan 20100820a.JPG

Amplified pSB1C3, pSB1T3, pSB1A3 accordingly to the table bellow.

Reagent Amount
DW 　34 uL
10x buffer 　5 uL
2mM dNTP 　5 uL
25mM MgSO4 　3 uL
Suffix-F 　0.5 uL
Prefix-R 　0.5 uL
KOD 　1 uL
DNA Template 　1 uL
Total 　50 uL
  • pSB1T3 failed to amplify
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