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Lab notes (9/6 - 9/12)

Contents 1 Lab notes (9/6 - 9/12) 1.1 Flagella Group 1.1.1 Restriction Digest of Gel Extracted FlhDCmut and pSB1C3 and pSB1AK3 1.1.2 Ligation of FlhDCmut and pSB1C3 1.1.3 Ligation of FlhDCmut and pSB1AK3

Flagella Group

Restriction Digest of Gel Extracted FlhDCmut and pSB1C3 and pSB1AK3

Date: 9/12 2010
Done By: Sheila
Protocol: RD1.1
Notes:
Restriction Digest mixtures
FlhDCmut and pSB1C3 Restriction digest
FlhDCmut

H2O	48μL
EcoR1	4μL
Pst1	4μL
FD Green buffer	8μL
Sample	20μL

pSB1C3

H2O	24μL
EcoR1	2μL
Pst1	2μL
FD Green buffer	4μL
Sample	10μL

FlhDCmut and pSB1AK3 Restriction digest
FlhDCmut

H2O	48μL
EcoR1	4μL
Spe1	4μL
FD Green buffer	8μL
Sample	20μL

pSB1AK3

H2O	24μL
EcoR1	2μL
Xba1	2μL
FD Green buffer	4μL
Sample	10μL

Results:

Ligation of FlhDCmut and pSB1C3

Date: 9/12 2010
Done By: Sheila
Protocol: LG1.2
Notes:
Results:

Ligation of FlhDCmut and pSB1AK3

Date: 9/12 2010
Done By: Sheila
Protocol: LG1.2
Notes:
Results: