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Contents 1 yneA 2 Arabinose-inducible promoter characterisation 2.1 Miniprep of BBa_I13401 (GFP coding sequence + terminator) 2.2 Double digests 2.3 Gel extraction of digests 2.4 Ligation

yneA

Nanodrop results:

1. 158.7
2. 261.2
3. 262.9
4. 204.7
5. 262.9
6. 202.1
7. 174.8
8. 226.6
9. 229.1
10. 164.5
11. 177.8
12. 225.1
13. 281.5
14. 249.3
15. 192.2
16. 239.3
17. 195.1

Arabinose-inducible promoter characterisation

...

Miniprep of BBa_I13401 (GFP coding sequence + terminator)

..from last night's overnight cultures from the 8th of september transformation.

Double digests

.. double digests for standard BioBrick assembly.. cut the arabinose-inducible promoter BioBrick with EcoRI and SpeI, cut gfp+terminator BioBrick with EcoRI and XbaI, for later ligation..

Gel extraction of digests

Ligation