Team:Newcastle/2 September 2010
From 2010.igem.org
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pH changing
We brought hepes (which is similar to homopipes) to pH 7 then autoclaved it.
Preparing medium for natto
We prepared antibitoic ,medium for natto: 17.5g of broth in 1L, so 1.75 in 100ml
Extreme Overnight cultures for Gibson
- G1 (T1) 1 Colony 1,
- G1 (T2) 7 Colonies 2-6
- G2 (T3) 2 Colonies 9,10
- G2 (T4) 14 Colonies 11-24
- G3 (T5) 3 Colonies 25-27
- G3 (T6) 2 Colonies 28-29
- G4 (T7) 4 Colonies 30-33
- G4 (T8) 9 Colonies 34-42
- G5 (T9) 0
- G5 (T10) 2 Colonies 43-44
Gibson for rocF
- Promoter 136bp - 0.7 microl
- rocF 1 246bp - 1.2 microl
- rocF 2 597bp - 1.9 microl
- rocF 3 125bp - 0.4 microl
- DTERM 116bp - 0.8microl
Total: 1190bp
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