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SEPTEMBER: WEEK 2
September, 6th
Inoculum of I47, I48, I49 into 5 ml LB+Amp for TECAN experiment of the following day.
- Inoculum of I44-2 and I51-3 into 5 ml LB+Cm34 to evaluate MiniPrep yields.
- Inoculum of I44-2 and I51-3 into 5 ml LB+Cm12,5 to evaluate MiniPrep yields and for sequencing.
- Inoculum for MiniPrep and sequencing of:
- <partinfo>BBa_J23100</partinfo>_4C5
- <partinfo>BBa_J23101</partinfo>_4C5
- <partinfo>BBa_J23105</partinfo>_4C5
- <partinfo>BBa_J23106</partinfo>_4C5
- <partinfo>BBa_J23110</partinfo>_4C5
- <partinfo>BBa_J23114</partinfo>_4C5
- <partinfo>BBa_J23116</partinfo>_4C5
- <partinfo>BBa_J23118</partinfo>_4C5
- I7-A
- I8-5-D
Inoculum of cultures for TECAN test on self-inducible promoters:
- I14-1
- I15-1
- I16-1
- I17-1
- I18-1
- I19-1
- I14_4C5-2
- I15_4C5-2
- I16_4C5-1
- I17_4C5-1
- I18_4C5-1
- I19_4C5-1
- I6-3
- I3-1
- <partinfo>BBa_B0031</partinfo>
September, 7th
MiniPrep for:
| Culture | Quantifications (ng/ul)
|
| <partinfo>BBa_J23100</partinfo>_4C5 |
|
| <partinfo>BBa_J23101</partinfo>_4C5 |
|
| <partinfo>BBa_J23105</partinfo>_4C5 |
|
| <partinfo>BBa_J23106</partinfo>_4C5 |
|
| <partinfo>BBa_J23110</partinfo>_4C5 |
|
| <partinfo>BBa_J23114</partinfo>_4C5 |
|
| <partinfo>BBa_J23116</partinfo>_4C5 |
|
| <partinfo>BBa_J23118</partinfo>_4C5 |
|
| I7-A |
|
| I8-5-D |
|
| I44-2 (Cm 34) |
|
| I51-3 (Cm 34) |
|
| I44-2 (Cm 12,5) |
|
| I51-3 (Cm 12,5) |
|
Cultures for TECAN test were diluted in the morning (h10:20) 1:100 (40ul in 4ml) and let grown at 37°C 220 rpm till they reach the desired OD.
September, 8th
September, 9th
September, 10th
September, 11th
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