Team:Calgary/Notebook/Safety And Protocols/Comments

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Safety and Protocols Main Page

Taq Polymerase Chain Reaction

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Transformable Competent Cell Induction

Bacterial Transformation

Restriction Digest

Ligation

Miniprep Plasmid Preparation (using GenElute)

Agarose Gel Electrophoresis

Making of Agarose Gels

Rehydration of Registry DNA

Construction Technique

Overnight Cultures

Glycerol Stock Preparation

LB Agar Plate Preparation

QIAprep Spin Miniprep Kit

Gel Extraction

PCR Purification

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