UTDallas/26 August 2010

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Notebook

June
MTWTFSS
  [http://2010.igem.org/wiki/index.php?title=UTDallas/1_June_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=UTDallas/2_June_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=UTDallas/3_June_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=UTDallas/4_June_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=UTDallas/5_June_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=UTDallas/6_June_2010&action=edit 6]
[http://2010.igem.org/wiki/index.php?title=UTDallas/7_June_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=UTDallas/8_June_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=UTDallas/9_June_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=UTDallas/10_June_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=UTDallas/11_June_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=UTDallas/12_June_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=UTDallas/13_June_2010&action=edit 13]
[http://2010.igem.org/wiki/index.php?title=UTDallas/14_June_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=UTDallas/15_June_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=UTDallas/16_June_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=UTDallas/17_June_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=UTDallas/18_June_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=UTDallas/19_June_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=UTDallas/20_June_2010&action=edit 20]
[http://2010.igem.org/wiki/index.php?title=UTDallas/21_June_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=UTDallas/22_June_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=UTDallas/23_June_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=UTDallas/24_June_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=UTDallas/25_June_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=UTDallas/26_June_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=UTDallas/27_June_2010&action=edit 27]
[http://2010.igem.org/wiki/index.php?title=UTDallas/28_June_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=UTDallas/29_June_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=UTDallas/30_June_2010&action=edit 30]
July
MTWTFSS
      [http://2010.igem.org/wiki/index.php?title=UTDallas/1_July_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=UTDallas/2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=UTDallas/3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=UTDallas/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=UTDallas/5_July_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=UTDallas/6_July_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=UTDallas/7_July_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=UTDallas/8_July_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=UTDallas/9_July_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=UTDallas/10_July_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=UTDallas/11_July_2010&action=edit 11]
[http://2010.igem.org/wiki/index.php?title=UTDallas/12_July_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=UTDallas/13_July_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=UTDallas/14_July_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=UTDallas/15_July_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=UTDallas/16_July_2010&action=edit 16] [http://2010.igem.org/UTDallas/17_July_2010 17] [http://2010.igem.org/wiki/index.php?title=UTDallas/18_July_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=UTDallas/19_July_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=UTDallas/20_July_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=UTDallas/21_July_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=UTDallas/22_July_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=UTDallas/23_July_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=UTDallas/24_July_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=UTDallas/25_July_2010&action=edit 25]
[http://2010.igem.org/UTDallas/26_July_2010 26] [http://2010.igem.org/UTDallas/27_July_2010 27] [http://2010.igem.org/UTDallas/28_July_2010 28] [http://2010.igem.org/UTDallas/29_July_2010 29] [http://2010.igem.org/UTDallas/30_July_2010 30] [http://2010.igem.org/wiki/index.php?title=UTDallas/31_July_2010&action=edit 31]
August
MTWTFSS
            [http://2010.igem.org/wiki/index.php?title=UTDallas/1_August_2010&action=edit 1]
[http://2010.igem.org/UTDallas/2_August_2010 2] [http://2010.igem.org/UTDallas/3_August_2010 3] [http://2010.igem.org/UTDallas/4_August_2010 4] [http://2010.igem.org/UTDallas/5_August_2010 5] [http://2010.igem.org/UTDallas/6_August_2010 6] [http://2010.igem.org/wiki/index.php?title=UTDallas/7_August_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=UTDallas/8_August_2010&action=edit 8]
[http://2010.igem.org/UTDallas/9_August_2010 9] [http://2010.igem.org/UTDallas/10_August_2010 10] [http://2010.igem.org/UTDallas/11_August_2010 11] [http://2010.igem.org/UTDallas/12_August_2010 12] [http://2010.igem.org/UTDallas/13_August_2010 13] [http://2010.igem.org/wiki/index.php?title=UTDallas/14_August_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=UTDallas/15_August_2010&action=edit 15]
[http://2010.igem.org/UTDallas/16_August_2010 16] [http://2010.igem.org/UTDallas/17_August_2010 17] [http://2010.igem.org/UTDallas/18_August_2010 18] [http://2010.igem.org/UTDallas/19_August_2010 19] [http://2010.igem.org/UTDallas/20_August_2010 20] [http://2010.igem.org/wiki/index.php?title=UTDallas/21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=UTDallas/22_August_2010&action=edit 22]
[http://2010.igem.org/UTDallas/23_August_2010 23] [http://2010.igem.org/UTDallas/24_August_2010 24] [http://2010.igem.org/wiki/index.php?title=UTDallas/25_August_2010&action=edit 25] [http://2010.igem.org/UTDallas/26_August_2010 26] [http://2010.igem.org/UTDallas/27_August_2010 27] [http://2010.igem.org/wiki/index.php?title=UTDallas/28_August_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=UTDallas/29_August_2010&action=edit 29]
[http://2010.igem.org/UTDallas/30_August_2010 30] [http://2010.igem.org/UTDallas/31_August_2010 31]
September
MTWTFSS
    [http://2010.igem.org/UTDallas/1_September_2010 1] [http://2010.igem.org/UTDallas/2_September_2010 2] [http://2010.igem.org/UTDallas/3_September_2010 3] [http://2010.igem.org/wiki/index.php?title=UTDallas/4_September_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=UTDallas/5_September_2010&action=edit 5]
[http://2010.igem.org/wiki/index.php?title=UTDallas/6_September_2010&action=edit 6] [http://2010.igem.org/UTDallas/7_September_2010 7] [http://2010.igem.org/UTDallas/8_September_2010 8] [http://2010.igem.org/UTDallas/9_September_2010 9] [http://2010.igem.org/UTDallas/10_September_2010 10] [http://2010.igem.org/wiki/index.php?title=UTDallas/11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=UTDallas/12_September_2010&action=edit 12]
[http://2010.igem.org/UTDallas/13_September_2010 13] [http://2010.igem.org/UTDallas/14_September_2010 14] [http://2010.igem.org/UTDallas/15_September_2010 15] [http://2010.igem.org/UTDallas/16_September_2010 16] [http://2010.igem.org/UTDallas/17_September_2010 17] [http://2010.igem.org/wiki/index.php?title=UTDallas/18_September_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=UTDallas/19_September_2010&action=edit 19]
[http://2010.igem.org/UTDallas/20_September_2010 20] [http://2010.igem.org/UTDallas/21_September_2010 21] [http://2010.igem.org/UTDallas/22_September_2010 22] [http://2010.igem.org/UTDallas/23_September_2010 23] [http://2010.igem.org/UTDallas/24_September_2010 24] [http://2010.igem.org/UTDallas/25_September_2010 25] [http://2010.igem.org/UTDallas/26_September_2010 26]
[http://2010.igem.org/UTDallas/27_September_2010 27] [http://2010.igem.org/UTDallas/28_September_2010 28] [http://2010.igem.org/UTDallas/29_September_2010 29] [http://2010.igem.org/UTDallas/30_September_2010 30]
October
MTWTFSS
        [http://2010.igem.org/UTDallas/1_October_2010 1] [http://2010.igem.org/wiki/index.php?title=UTDallas/2_October_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=UTDallas/3_October_2010&action=edit 3]
[http://2010.igem.org/wiki/index.php?title=UTDallas/4_October_2010&action=edit 4] [http://2010.igem.org/UTDallas/5_October_2010 5] [http://2010.igem.org/UTDallas/6_October_2010 6] [http://2010.igem.org/UTDallas/7_October_2010 7] [http://2010.igem.org/UTDallas/8_October_2010 8] [http://2010.igem.org/UTDallas/9_October_2010 9] [http://2010.igem.org/wiki/index.php?title=UTDallas/10_October_2010&action=edit 10]
[http://2010.igem.org/UTDallas/11_October_2010 11] [http://2010.igem.org/UTDallas/12_October_2010 12] [http://2010.igem.org/UTDallas/13_October_2010 13] [http://2010.igem.org/UTDallas/14_October_2010 14] [http://2010.igem.org/UTDallas/15_October_2010 15] [http://2010.igem.org/UTDallas/16_October_2010 16] [http://2010.igem.org/UTDallas/17_October_2010 17]
[http://2010.igem.org/UTDallas/18_October_2010 18] [http://2010.igem.org/UTDallas/19_October_2010 19] [http://2010.igem.org/UTDallas/20_October_2010 20] [http://2010.igem.org/UTDallas/21_October_2010 21] [http://2010.igem.org/UTDallas/22_October_2010 22] [http://2010.igem.org/UTDallas/23_October_2010 23] [http://2010.igem.org/UTDallas/24_October_2010 24]
[http://2010.igem.org/UTDallas/25_October_2010 25] [http://2010.igem.org/UTDallas/26_October_2010 26] [http://2010.igem.org/wiki/index.php?title=UTDallas/27_October_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=UTDallas/28_October_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=UTDallas/29_October_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=UTDallas/30_October_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=UTDallas/31_October_2010&action=edit 31]
November
MTWTFSS
[http://2010.igem.org/wiki/index.php?title=UTDallas/1_November_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=UTDallas/2_November_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=UTDallas/3_November_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=UTDallas/4_November_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=UTDallas/5_November_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=UTDallas/6_November_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=UTDallas/7_November_2010&action=edit 7]
[http://2010.igem.org/wiki/index.php?title=UTDallas/8_November_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=UTDallas/9_November_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=UTDallas/10_November_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=UTDallas/11_November_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=UTDallas/12_November_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=UTDallas/13_November_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=UTDallas/14_November_2010&action=edit 14]
[http://2010.igem.org/wiki/index.php?title=UTDallas/15_November_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=UTDallas/16_November_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=UTDallas/17_November_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=UTDallas/18_November_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=UTDallas/19_November_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=UTDallas/20_November_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=UTDallas/21_November_2010&action=edit 21]
[http://2010.igem.org/wiki/index.php?title=UTDallas/22_November_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=UTDallas/23_November_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=UTDallas/24_November_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=UTDallas/25_November_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=UTDallas/26_November_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=UTDallas/27_November_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=UTDallas/28_November_2010&action=edit 28]
[http://2010.igem.org/wiki/index.php?title=UTDallas/29_November_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=UTDallas/30_November_2010&action=edit 30]


August 26, 2010

Today we redid the PCR for Pu since it did not work on Tuesday. One of the sets of primers worked. I am also attaching all of the gel pictures that we have so far. They are not great quality, so if you can't see something that should be there just email me back.

The pictures are named based on the date the lab was. Under this I am putting additional information about what each picture is showing (from the left side of the photo to the right side). They are all from gel electrophoresis.

8-16 There is a 2-log DNA ladder, then there is not a correct band for Pu, then there is a band for PyeaR

8-16(2) There is a 2-log DNA ladder, then Pr+XylR correct, then Terminator incorrect, then Red correct, then GFP correct, then Orange correct, then mRFP1 correct, and then a 1 kb DNA ladder

8-17 There is a 2-log DNA ladder then a PyeaR band, it is correct

8-20 This is again a 2-log DNA ladder and then PyeaR, correct

8-20 (2) This is the double terminator and then the 1 kb DNA ladder (The gel messed up which is why the ladder is hard to read, but the terminator is probably correct)

8-23 This is the gel ran after the first Pu PCR attempt. There is a 1 kb DNA ladder (should have used the 2-log DNA ladder), then the Pu band, which looks correct

8-24 There is a band for Pu that is incorrect and then a 2-log DNA ladder

8-26 We tried four different combinations of primers for the Pu PCR and this is the gel with the results. First is the 2-log DNA ladder, then primers 1&2 incorrect, primers 1&4 maybe, primers 3&2 incorrect, and primers 3&4 correct.

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