"

From 2010.igem.org

Contents 1 Andreas 1.1 Cloning of SOD into pMA.His 1.1.1 Transformation results 1.1.2 Colony PCR 1.1.3 Gel verification 1.1.4 ON cultures

Andreas

Cloning of SOD into pMA.His

Transformation results

From 28 28/8 Good colony yield. Four colonies (SH1-SH4) picked for colony PCR.

Colony PCR

• SH1-SH4: pMA.SOD⋅His
• PC: Positive control; pMA.His
• NC: Negative control; blank

Procedures according to standard colony PCR protocol. Elongation time 1:00.

Gel verification

1 % agarose, 100 V

Expected bands:

• pMA.SOD⋅His: 831 bp
• pMA.His: 348 bp

Results
Well corresponding bands indicating successful insertion of SOD into the vector.

ON cultures

SH1 and SH2 selected for plasmid prep and sequencing. Set 5 ml LB + 100 Amp ON cultures. 37 °C, 225 rpm.