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Team:Newcastle/23 August 2010
From 2010.igem.org
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Contents |
pSB1C3 plasmid gel electrophoresis
Aims
The aim of this experiment is to run gel electrophoresis for the extracted and linearized plasmid pSB1C3 fragment which were amplified at 4 different melting temperatures by 4 separate PCR reactions.
Materials and Protocol
Please refer to: Gel electrophoresis for gel electrophoresis protocol.
Result
Figure 1: Gel electrophoresis of plasmid pSB1C3 linearized fragments which are amplified by 4 different PCR reactions running at 4 different melting temperatures.
- Lane 1: 1 kb DNA ladder
- Lane 2: pSB1C3 fragment amplified at 55°C
- Lane 3: pSB1C3 fragment amplified at 55°C
- Lane 4: pSB1C3 fragment amplified at 60°C
- Lane 5: pSB1C3 fragment amplified at 60°C
- Lane 6: pSB1C3 fragment amplified at 65°C
- Lane 7: pSB1C3 fragment amplified at 65°C
- Lane 8: pSB1C3 fragment amplified at 70°C
- Lane 9: pSB1C3 fragment amplified at 70°C
- Lane 10: 1 kb DNA ladder
Discussion
Conclusion
   
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