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From 2010.igem.org

Formal Meeting 26th May 2010

1. Roll call and apologies

1. Approval of agenda and minutes

1. Brief summary of previous meeting

1. Research Feedback

1. 1. Biobrick 1 (CaCO3/ Urease)

Biobrick tutorial taught last to last week by Anil on Urease. Urease has a complicated genetic circuit. Should start forming questions which would help in building the egnetic circuit of the biobrick.

1. 1. Biobrick 2 (End of crack and signalling system)

Need to search for the peptides which are to be used for quorum sensing.

1. 1. Biobrick 3 (Non target environment kill switch)

There are not many biobricks in the iGEM registry on bacterial lysis. There is a lot of data on lysis by Richard on the wiki. WE should start looking up for ftsZ and SOS system for lysis and a biobrick can be made early based on this and can be characterized. Need to look up on ftsZ paper. Bacterial Time Bomb.

1. 1. BioBrick 4 (Spider silk & filamentous cells)

Read the 2 papers sent by Anil on glue and spider silk.

1. 1. BioBrick 5 (glue and lysis)

1. 1. BioBrick 6 Sequential Programming

Modelling is almost at an end. (Richard)

1. Visa/Passport update

Harsh called but didn't get any dates for the month of June so will be applying in July again. Send visa draft document to Anil- Harsh

1. Travel plans

Too early to book flights and hotels. May have other members joining.

1. Tutorial

Formal demonstration of Clone Manager next week - Anil

1. Flow Chart

Media:Flowchart_overview-1-.ppt‎ There are 3 sensing systems:

• Are the cells at an optimal density?
• Sensing system for glue production
• End of crack signalling

1. Timeline (Janetta)

Put timeline up on the current wiki.

1. Other business

None

1. Action points
   1. Send the email to Colin Harwood (Richard)
   2. Someone ask Anil to put the powerpoint presentation of the tutorial in the dropbox
   3. Booking for the rooms - Jen
   4. Rolling booking has been made for CBCB building for every Wednesday - Anil
   5. New biobricks have arrived
   6. Wendy a post doc and a molecular bilogist would be working with us in the lab and would be guiding us.
   7. Lab training will start from the end of June- Anil
   8. For the lab trainig the current team will be split into 2 teams and the training would last for 2 weeks where in the 1st week one team would be undergoing their training and the other team would be designing biobricks and in the 2nd team the tasks will be switched between the 2 teams.
   9. Need to plan out what the other team would exactly be doing within that 1 week.
   10. Before the end of June we would be making the design of the biobrick.
   11. Google up for the video on the tutrial for Cell Designer and Kapasi.
   12. Good News: Goksel is now an official advisor of the iGEM team 2010 and would be helping us with modelling and designing the biobricks.
   13. Need to look up on the registry for the biobricks which are used before, which are new and which one needs optimizing- everyone.
   14. Move Da from biobrick 2 to biobrick 3.
   15. Add Goksel to iGEM 2010 list - Jen.

1. Next formal meeting is at CBCB at 4.00 pm

1. Chair-Harsh 2. Minutes-Rachel 3. Computer-

Minutes for formal meeting 1st April 2010

* Room: Claremont 922; Start: 3 pm

* Chair: Rachel, Minutes: Harsh, Computer: Rachel

Apologies - Steven, Younus, Da, Alan, Phil

Approved