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Contents 1 LacI BioBrick Construction 1.1 Aims 1.2 Materials 1.3 Protocol 1.4 Results

LacI BioBrick Construction

Aims

• To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).

Materials

• Second round of Transformants
• Plasmid extracts
• Restriction enzymes Xba1 aand Pst1

Protocol

Single and Double digest of Plasmid extracts (single Xba1) Run digests on a gel Overnight culture of second round of transformants

Results