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Contents 1 Transformation 1.1 Results/Aims 2 Ligation 2.1 Aims

Transformation

Results/Aims

• pVeg transformed sucessfully- From this we will set up an overnight broth culture for plasmid extraction
• lacI- To show that we have sucessfully transformed form lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.

Ligation

Aims

Today we will transform some competent E.coli DH5alpha with the ligation products: 1:5,1:3 and the vector