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Screening for lacI insert

Aim

The aim of this part of this experiment is to screen for the lacI insert.

Protocol

• 1. Miniprep - the miniprep protocol was followed.
  2. Restriction digests - the restriction digests protocol was followed to allow the length of the DNA to be checked using single and double digest.
     • Single digest with Pst1
     • double digests with Pst1 and Xba1
  3. Gel electrophoresis - the gel electrophoresis protocol was followed.

Inference

• Set up liquid broth culture in LB