Tuesday July 20, 2010

Gel electrophoresis of Raida's PCR products: BBK primers that anneal before the multiple cloning sites. Lane 1 and 2: Lux0047 A + B0015 psB1AK3 Lane 3: J23002 + Lux0047E, Lane4: R0040 + I3502, Lane 5: Lux0047E, Lane 6: Master Mix

Raida

Today I set up and ran two 1% gels: one for my PCR products from yesterday and the second one for Emily's PCR products- both of which were done to test whether our primers are working or not. Please see the gel to the side: As we can see in the gel electrophoresis image, the expected results has been obtained. For example, Lane 5 contains only the Lux0047E gene where as Lane 3 has J23002 + Lux0047E, hence the band in Lane 5 goes slightly further relative to Lane 3 because it is a shorter band. Lanes 1 and 2 show exactly the same band because they are the same genes in the same plasmid backbone. Furthermore, Lane 6 shows no band because it is the Master Mix and our negative control. Therefore, it can be concluded that the primers are functional.