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Dr. E. coli : 世界最小の注射器

   私たちのマインテーマは三型分泌装置という菌が持つ器官の中でも驚くべきものだと思います。この注射器に似た装置はタンパク質を菌の中から標的の真核細胞に打ち込むものです。しかしこの装置は病原性グラム陰性菌であるヤルシニアやサルモネラの器官です。私たちが非病原性の大腸菌を用いてそれを安全使えることを目指しました。

PCR Based Assembly Protocol

   時々、スタンダードプロトコールではプラスミドが増えなかったり、BioBrickがホストセルに有害で増幅されないことがある。こんな時にPCRはすごく役立つのである。

Sometimes standard protocol fails to produce plasmid or BioBrick is toxic to host cell and can't be amplified. In moments like these PCR is handy. We think we might slightly improved it. You no longer have to guess if restriction was successful.

Team

   The Hokkaido University's igem 2010 team, HokkaidoU_Japan is Japans 8th Our has one instructer and 7 undergrad students.They come from faculty of Sciences, Medicine and Agriculture. This is the first year we are participating in iGEM.

Notebook

    The record of our triumphs and failures. Well big part of it is failures. But we did it.

Protocols

    General protocols we used.

Parts

   We registered two parts associated with main project. One is a signal sequence that can be used to secrete tagged proteins through Type III Secretion Apparatus. The other is whole reporter construct to checks whether the Type III secretion system works correctly.

   Also, we registerd one primer set which is useful for the PCR based protocol.

Safety

   Addressing bio safety concerns

Acknowledgements

Laboratory and Personnel

• Ken-ichi Yamazaki
  • Associate Professor/Environmental Molecular Biology/Graduate School of Environmental Earth Science/Hokkaido University
• Hideaki Higashi
  • Associate Professor /Division of Molecular Oncology/ Institute for Genetic Medicine/ Hokkaido University
• Hisatoshi Shida
  • Professor/Molecular Biology/Institute for Genetic Medicine/ Hokkaido University
• Takashi Oohashi
  • Associate Professor /Division of Molecular Oncology/ Institute for Genetic Medicine/ Hokkaido University
• Members of Yamazaki Lab
• Masaaki　Morikawa
  • Professor/Environmental Molecular Biology/Graduate School of Environmental Earth Science/Hokkaido University
• Hidetoshi Okuyama
  • Associate Professor/Environmental Molecular Biology/Graduate School of Environmental Earth Science/Hokkaido University

Thank you for letting us use some of the lab equipment.

Academic Institutions

• Salmoneｌｌa Genetic Stock Center/University of Calgary
  • Provided E.coli with BAC vector library coding for Type 3 Secretion Apparatus and SlrP signal
• Graduate School of Environmental Earth Science
• Department of biological science/School of Sciense
• Institute for Genetic Medicine

Companies

• Amino Up Chemical
• ECONIXE
• Cosmo Bio
• Mendel　Workshop

Person

• Tadasu Emoto
  • Manager of ECONIXE