Team:Heidelberg/Modeling/miGUI

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miRNA Binding Site

Engineering and Assembly Tool

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GUI_Picture shRNAmir Promoter_1 Promoter_2 Operator miGene shR_BS miR_BS Reference tet-R_Promoter tet-Repressor tet-miR_BS Virus

shRNAmir:
Promoter 1:
Promoter 2:
Operator:
miGene:
shR_BS:
miRNA

Knockdown

miRNA_BS:
Target:

Off-Target:

On: Off:
Treshold:
Reference:
tet-Promotor:
tet-Repressor:
tet-miR_BS:
Virus:

Give shRNAmiR

Choose your first promoter

Choose your second promoter

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Variable3

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Variable4

Please enter your target miRNA and the desired knockdown

miRNA

Knockdown

miRBS

Choose your Target:

Choose your Off-Targets:

On-Targeting Off-Targeting

Set relative miRNA treshold

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Variable6

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Variable7

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Variable8

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Variable9

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Variable10

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