"

From 2010.igem.org

Blue Light Producer

5/7/2010

• LuxAB & LuxCDE operon agarose gel
• Lane1: About2.1.kbp
• Lane 2: The highest band- very faint- about 3.5kbp

13/7/2010

Empty edinbrick vector for control.

• LuxAB + edinbrick ligation.

28/7/2010

an update on LuxAB:

• The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
• Still have some digested LuxAB-ediI so re-did ligation and transformation.

Lane 1&15	Lane 2	Others
Ladder	EdiI	LuxAB

Lane 1	Lane 2 +3	Lanes 3-6
Ladder	EdiI	LuxAB

29/7/2010

• The ligation had the same gel characteristics as previous luxAb-ediI ligations, e.x. it isn't visivle- not hopeful of this transformation working

4/8/2010

• made minipreps of blue (negative colonies)- double digest and ran in comparison with pure edinbrickI
• LacZ band dissapears but colonies still grow blue (WTF)
• Has bands equaling to luxAB and EdiI but there was no glowing

12/8/2010 update on LumP:

• still getting pink/red colonies from transformants
• single digests give a band of about 3kb
• double digests give a band of about 2.5 kb -highly confusing
• and also, no sign on RFP despite red/pink growth.
• Vector wa ssequenced and LumP is definately there.

Lanes 2-5: single digests of lumP Lane 6: double digest of lumP

26/8/2010

• Marker (Lane 1), minipreps 6-10 luxABlumP (lanes 2-6)

• single digests of LuxABlumP

7/9/2010

• minipreps of luxCDE: samples 1&4 usable

Lane 1	Lane 2-6	Lanes 7
Ladder	luxCDE00:1-5 minipreps	Ladder