Team:Queens-Canada/19 July 2010

Gel Electrophoresis

Yuli

Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.

Gel Electrophoresis

Hao

pSra-10

Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.

Liquid Culture

Hao

pSip-1::1A3 (Amp), BBa_I13522

Diagnostic Digestion

Yuli

pSip-1::1A3

Cut with: XbaI, SpeI, and PstI or NotI, EcoRI.

Gel Electrophoresis

Yuli and Chris

pSip-1 Digests

Used 0.3 g agarose in in 50 mL TBE. Gel run for 45 mins at 100 V.

Microinjection

Hao

Practiced injections with water