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Team:Queens-Canada/19 July 2010
From 2010.igem.org
July 19, 2010
Gel Electrophoresis
Yuli
Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.
Gel Electrophoresis
Hao
pSra-10
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.
Liquid Culture
Hao
pSip-1::1A3 (Amp), BBa_I13522
Diagnostic Digestion
Yuli
pSip-1::1A3
Cut with: XbaI, SpeI, and PstI or NotI, EcoRI.
Gel Electrophoresis
Yuli and Chris
pSip-1 Digests
Used 0.3 g agarose in in 50 mL TBE. Gel run for 45 mins at 100 V.
Microinjection
Hao
Practiced injections with water.
