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From 2010.igem.org

Lab Work

We harvested the 50 mL bacterial cells of the 14 BioBricks. We used 3 mL of the baceterial cells to make -80 °C stocks. With the rest we performed a Qiagen Midi-prep plasmid isolation.

The following plasmid concentrations were obtained:

BioBrick	Concentration (ng/μL
B0015	360.5
B0032	80.4
B0034	181.7
E0040	66.0
E0240	305.6
E0422	411.0
J61100	128.7
J61101	167.7
J61107	195.9
J61117	62.7
J61127	33.8
R0010	40.0
R0011	42.0

Pseudomonas Putida GPO1 is growing 1 day on different substrates. We measured absorbance of the Pseudomonas Putida to see on which conditions the strain survives. Absorbance measurements