Talk:Team:IvyTech-South Bend/21 September 2010

From 2010.igem.org

9/21/10

Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23

1) I will put frozen electro comp cells from -80 degrees C freezer

2) We will do two “2” electroporations with (AT) one with 5 mL T9002 Gene, and one with 20 mL

3) Icing Cuvette (.1) and now prepared to electroporate

4) Reading for 20 mL was 2.2 kv @ 0.70 ms

5) then removed with 1 mL SOB media

6) Placed into 1.5 mL Sterile Centrifuge tube at room temp for 3 hrs prof T. will streak.