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From 2010.igem.org

9/21/10

Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23

1) I will put frozen electro comp cells from -80 degrees C freezer

2) We will do two “2” electroporations with (AT) one with 5 mL T9002 Gene, and one with 20 mL

3) Icing Cuvette (.1) and now prepared to electroporate

4) Reading for 20 mL was 2.2 kv @ 0.70 ms

5) then removed with 1 mL SOB media

6) Placed into 1.5 mL Sterile Centrifuge tube at room temp for 3 hrs G T. will streak.

JH

9/21/10

Pulling of LacZ plate 1 well 23F __ I732094 I added 10ul of di H2O to the well, did not pull 5 ul out because we did not have electorcom cells ready There is 10 ul of di H20 and lacZ in well --Rchamberlin 18:49, 26 October 2010 (UTC) for Dgarvey