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From 2010.igem.org

October 10 (Sun)

1. Electrophoresis
2. Ligation
   • 048(C) R1 + FcenA
   • 060(C) 001-2 + GeneⅢ
   • 051(C) R2 + 022
   • 066(A) 3-2P + 026
   • 069(A) 049 + 026
   • 070(A) 052 + 026
3. mini prep
   • 058①,051②,016①,013②
4. cut check
   • 016①,058①,013②,051②,1-13D,050①,025②
   • PCR Products(ADH2,ENO1)
   • Man→034
   • glr→031
   • ADH2→027
   • ENO→029
   • Cel44A→075

1. PCR for DNA segment
   • Cel5B 3/4 α-agultinin
2. To liquid culture
   • 1-13D,1-12M,1-23L,013,056,069-2,061 →culture
3. Transformation
   • 048,060,051,066,069,070,1-13D

October 11 (Mon)

1. Transformation
   • 034,031,027,029,075
   • 014-2,021-2,022-2,025-2,047
2. mini prep
   • 1-23L,1-12M,061①,064-2②,056①②,013①②
   • 1-13D,061②,064-2① →　destruction
3. cut check
   • 1-12M,064-2②,013①,056①,061①,1-23L
4. To liquid culture
   • plate 025(10/8),022(10/8) each 2 colony to culture
5. Electrophoresis
   • 064 ok
   • 061 x
   • 056 x
   • 013 ok
6. Ligation
   • 048(C) R1 + FcenA
   • 051(C) R2 + FcenA
   • 053(C) R2 + 024
   • 059(C) 001-2 + ToR
   • 068(K) 050 + 026
   • 070(A) 052 + 054
   • 072(K) 049 + 054
   • 076(A) 050 + 054
   • 077(A) 2-4A + R1
7. cutcheck
   • R2 →XP
   • 1-23L→ E
8. To liquid culture
   • 1-13D①,070①②,066①②,069①②,060①②,048①②,051①②
9. Electrophoresis
   • 1-23L ok
   • 036② ok
10. Ligation
    • 086(C) 024 + 1-23L
    • 078(A) 2-4A + R2
    • 085(C) 023 + 1-23L
    • 084(K) 049 + 1-23L
    • 083(K) 3-2P + 1-23L
    • 082(K) 035 + 1-23L
    • 081(C) 022 + 1-23L
    • 079(C) 036 + 1-23L

October 12 (Tue)

1. mini prep
   • 1-13D,022-2①②,048①②,060①②,066①②,069①②,070①②
   • 051①→　destrustion
2. Transformation
   • 047,063,069,0071,078,079,080,081,082,083,084,085
3. cutcheck
   • 1-13D,022-2,048,066,069,070,1-13D,060
4. Electrophoresis
   • 1-13D →x
   • 1-13 →x
   • 022-2 →x
   • 048 →OK
   • 060 →?
   • 066 →OK
   • 069 →OK
   • 070 →OK
5. To liquid culture
   • 034,029,027,019-2,022-2,025-2,021-2,031,075,051,072,070,068,053,059,077,076,048,060,1-23L
6. cutcheck
   • 1-23L,048
7. Electrophoresis
8. .Ligation
   • 030(C) SUC d + 1-3A
   • 087(A) 048 + 026
   • 088(A) 048 + 054
   • 089(C) 2-4A + 054

October 13 (Wed)