Team:Tsinghua/Notebook/9 October 2010

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Module I, Group 2c

After purification, ligation reaction took place in a mixed system as following， respectively. 

eGFP                 3ul
PSB1C13              2ul
T4 ligase(NEB)       1ul
T4 ligation Buffer   2ul
H2O                  12ul

mCherry              2ul
PSB1C13              2ul
T4 ligase(NEB)       1ul
T4 ligation Buffer   2ul
H2O                  13ul

Kan                  3ul
PSB1C13              2ul
T4 ligase(NEB)       1ul
T4 ligation Buffer   2ul
H2O                  12ul

Incubate at 16 centidegree overnight.

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