Team:Tsinghua/Notebook/8 October 2010

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Module I, Group 2c

Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I.


Double digestion system

eGFP                 5ul
EcoR I               1ul
Pst I                1ul
FD Buffer            2ul
H2O                  11ul

mCherry              4ul
EcoR I               1ul
Pst I                1ul
FD Buffer            2ul
H2O                  12ul
----
Kan                  4ul
EcoR I               1ul
Pst I                1ul
FD Buffer            2ul
H2O                  12ul

Incubate at 37 centidegree overnight.