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Team:Chiba/lux promoter
From 2010.igem.org
| Lux promoter inverter We've charaterized Plux inverter. There is promoter (HSL-luxR repressor), biobrick number R0061. We've prepare Plux inv-GFP and characterized about it. R0061の下流にRBSおよびGFP遺伝子 (E0240) を挿入したプラスミド (Plux inv-GFP) 作製し,LuxRの抑制機能を調べた |
| Experiments 1.Preparing Plux inv-GFP Preparing Plux inv-GFP process is shown in '''Fig. 1'''.GFPの蛍光が確認されたので，Plux inv-GFPは機能している。 and sequence is conformed. |
Fig. 1 cloning process
| 2.Characterization Plux inv-GFP |
| Results Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。 |
Fig. 2 assay process
| Reference 1) Egland.K.A, and Greenberg.E.P, Conversion of the ''Vibrio Fischeri'' Transcriptional Activator,LuxR, to a Repressor, ''J. Bacteriol.'', '''182''', P.805-811 (2000) 2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, ''Mol Syst Biol'', '''3''' ,145 (2007) |
