From 2010.igem.org
|
|
|
Gel electrophoresis
Materials
- 50X TAE buffer
- Make up 1 liter of 50X TAE buffer with the following:
- 242 g of TRIS base
- 57.1 ml of acetic acid
- 100 ml of 0.5 M of EDTA (pH 8.0)
- Top up to 1 liter with water
- SafeView
- Agarose
- DNA ladder
- Eppendorf
- Gel making tank
- Gel running tank
Procedure
- Make up 1% agarose gel (1 g of agarose per 100 ml of TAE buffer) and using the microwave to dissolve the agarose.
- Allow the molten agarose to cool down before adding 5 μl of Safeview dye.
- Set up the gel tray containing the gel comb and transfer 60 ml of molten agarose gel into the gel tray (take care not to cover the gel comb).
- Wait for 30 min to allow the gel to harden.
- Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerged.
- Depending on the nature of the sample, 3 μl of GeneRuler™ 1 kb Plus DNA Ladder is used for analysing DNA.
- Loading buffer is then added together with the sample before loading onto the gel matrix.
- Run gel at 90V until the desired separation is achieved and visualize using the gelDoc.
Go back to our Protocol List
"
