Team:TU Delft/21 June 2010 content

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We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.

Lab work

Restriction digestion of plasmid backbone pSB1T3:

pSB1T3 (~1,0 μg)	2,5 μL
Buffer H (Roche)	2,0 μL
EcoRI (1 U/μg)	1,0 μL
PstI (1 U/μg)	1,0 μL
H2O	13,5 μL

Cut digested pSB1T3 from gel and isolate the band.