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Contents 1 Characterization of yneA 1.1 Aim 1.2 Materials and Protocol 1.3 Result and Conclusion

Characterization of yneA

Aim

The aim of this experiment is to prepare slides for the Bacillus subtilis 168 cells which have yneA integrated into the chromosome or is on the plasmid (pMutin4 and pMap65) and are induced by IPTG at various concentrations.

Materials and Protocol

Please refer to: Slide preparation for IPTG induced cells for materials and protocol. For this experiment we used the following cell colonies:

1. Bacillus subtilis 168
2. Bacillus subtilis 168 with pMutin4 having yneA insert
3. Bacillus subtilis 168 tith pMap65 having yneA insert

Grow them overnight.

Result and Conclusion

Please refer to: [[]] for result and conclusion of the experiment.