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Team:Newcastle/3 September 2010
From 2010.igem.org
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Contents |
yneA
Aims
The aim of the experiment is to test for the correct integration of yneA in pGFPrrnb plasmid.
Materials and protocols
- Please refer to: Plasmid extraction.
- Please refer to:Restriction digest. We used Nhe1 and Spe1 to remove the yneA from pGFP-rrnB.
- Please refer to: Gel electrophoresis for running all the digested plasmid fragments.
Results
Figure 1:
- Lane 1: 1kb DNA ladder
- Lane 2: Plamid pMutin4 containing lacI
- Lane 3: Plamid pMutin4 containing Pspac_oid promoter
- Lane 4: 100bp DNA ladder
- Lane 5:
- Lane 6:
- Lane 7:
- Lane 8:
- Lane 9:
- Lane 10:
- Lane 11:
- Lane 12:
- Lane 13:
- Lane 14: 1kb DNA ladder
Conclusion
The results show that the digest works, there is a band at approximately 541bp corresponding to yneA and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.
   
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